研究方向：

1. 微生物学研究的新技术和新方法

2. 资源环境微生物

3. 微生物天然产物

学习、工作经历：

2002年9月-2006年7月 北京大学，元培学院，获化学学士学位

2006年9月-2011年7月 北京大学，化学分子与工程学院，获化学生物学博士学位

2011年7月-2013年6月 清华大学，化学系，博士后

2013年6月-2014年12月 北京林业大学，生物科学与技术学院，讲师

2015年1月-2023年12月 北京林业大学，生物科学与技术学院，副教授

2017年3月-2018年3月 美国Wisconsin-Madison，药学院，访问学者

2024年1月至今 北京林业大学，生物科学与技术学院，教授

奖励及荣誉：

1. 北京林业大学第十五届青年教师教学基本功比赛二等奖（2019）

2. 北京市大学生生物学竞赛实验设计竞赛二等奖指导教师（2019）

3. 北京林业大学教育教学研究论文一等奖（2019）

4. ISW国际认证证书

承担项目：

1. 基于微流控芯片的油蒿根际微生物组高通量培养鉴定及促生功能研究，2022-2025，国家自然科学基金面上项目，主持

2. 杨树丛枝菌根真菌的多样性分析及离体纯培养体系探索，2021-2022，北京林业大学科技创新计划项目，主持

3. 微流控芯片质谱联用在植物内生菌培养组学研究中的应用，2018-2021，国家自然科学基金面上项目，主持

4. 微流控技术在微生物细胞共培养中的应用，2019-2020，北京林业大学科技创新计划项目，主持

5. 基于微流控液滴的放线菌高通量筛选培养和分离，2015-2017，国家自然科学基金青年基金，主持

6. 植物内生放线菌次生代谢产物的发掘及其抗癌新机制研究，2013-2014，北京林业大学新进教师科研启动基金项目，主持

7. 木质素生物解聚及其机制研究，2017-2019，北京林业大学科技创新计划项目，参与

8. 基于纸基电喷雾的多通道微流控芯片质谱联用装置的研制，2013-2016，国家自然科学基金专项基金项目，参与

9. 微流控液滴包覆细胞新方法研究及其在药物筛选中的应用，2014-2017，国家自然科学基金面上项目，参与

10. 基于化学小分子研究STAT3与心力衰竭相关靶基因RLX的相互作用及信号转导的调控规律，2009-2013，国家自然基金重大研究计划培育项目，参与

发表文章情况：

1. Zhu W., Han S., Cheng Y., Yu Z., Zhao G., He, X.*, Root zone microbial communities of Artemisia ordosica Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights, Frontiers in Microbiology. 2025,16:1585700.

2. Li Y., Han S., He X.*, High-throughput sequencing reveals microbial community dynamics in two treatment systems for oil field-produced water, Canadian Journal of Microbiology. 2025, 00:1-11

3. Zheng F., Yang J., Luo H., Sun Q., Zhang X., Li R., He X.*, Zhao G.*, Hydrolysis Mechanism of Multimodular Endoglucanases with Distinctive Domain Composition in the Saccharification of Cellulosic Substrates, Biomacromolecules. 2024, 25(9):6007-6016

4. Wu G., Chen J., Dou J., He X.*, Li H.*, Lin J., An electrochemiluminescence microsensor based on DNA-silver nanoclusters amplification for detecting cellular adenosine triphosphate, Analytical Methods. 2024, 16(14):2019-2024.

5. Han, S.; Cheng, Y.; Wu, G.; He, X.*; Zhao, G.*, Enhancing Salt Tolerance in Poplar Seedlings through Arbuscular Mycorrhizal Fungi Symbiosis. Plants (Basel, Switzerland). 2024,3, 233

6. Yang, J.; Zhang, X.; Sun, Q.; Li, R.; Wang, X.; Zhao, G.; He, X.; Zheng, F.*, Modulation of the catalytic activity and thermostability of a thermostable GH7 endoglucanase by engineering the key loop B3. International Journal of Biological Macromolecules. 2023, 248

7. Wang, X.; Wu, G.; Han, S.; Yang, J.; He, X.*; Li, H.*, Differentiation and Identification of Endophytic Bacteria from Populus Based on Mass Fingerprints and Gene Sequences. International Journal of Molecular Sciences. 2023, 24(17)

8. Cheng, Y.; Zhu, W.; Han, S.; Yang, J.; Wu, G.; Zhao, G.; He, X.*, Roseomonas populi sp. nov., an acetate-degrading bacteria isolated from the stem of Populus tomentosa. Antonie van Leeuwenhoek. 2023, 117(2)

9. Shen, L.; An, M.; Liang, R., Li, Y., He, X., Zhao, G.*, Polyphase taxonomy and genome analysis reveal the adaptability of Luteolibacter rhizosphaerae sp. nov. to the rhizosphere soil of Ulmus pumila L. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2023, 116(8):1-10

10. Han, S.; Wang, X. Cheng, Y.; Wu, G.; Dong, X., He, X.*; Zhao, G.*, Multidimensional analysis reveals environmental factors that affect community dynamics of arbuscular mycorrhizal fungi in poplar roots. Frontiers in plant science. 2023, 13

11. Shen, L.; An, M.; He, X., Zhao, G.*. Luteimonas galliterrae sp. nov., isolated from poultry farm soil. International Journal of Systematic and Evolutionary Microbiology. 2023, 73(2)

12. Shen, L.; Liu, P.; An, M.; Liang, R; He, X.; Zhao, G.*, Sphingomonas quercus sp. nov., Isolated from Rhizosphere Soil of Quercus mongolica, Current microbiology. 2022, 79(4), 122

13. Shen, L.; An, M.; Liang, R., Liu, P., He, X., Zhao, G.*, Roseococcus pinisoli sp. nov., lacking pufL and pufM bacteriochlorophyll a: synthesizing genes, Archives of Microbiology. 2022, 204(6)

14. Shen, L.; Liu, J.; Liu, P.; An, M.; He, X.; Zhao, G.*, A non-symbiotic novel species, Rhizobium populisoli sp. nov., isolated from rhizosphere soil of Populus popularis, Archives of Microbiology. 2022, 204(1)

15. 杨敬敬, 韩烁, 王霞, 赵国柱, 何湘伟*, 三倍体毛白杨不同组织内生细菌多样性分析, 微生物通报. 49(8), 3021-3036, 2022

16. Ma, J., Cai, C., Liu, J.., Gao, S., Zhao, G.*. He, X.. In Vitro Antibacterial and Antitumor Activity of Total Triterpenoids from a Medicinal Mushroom Sanghuangporus sanghuang (Agaricomycetes) in Liquid Fermentation Culture, International Journal of Medicinal Mushrooms. 2021, 23(7), 27-39

17. Chen, J.; Li, H. F.*; Zhao, G.; Lin, J. M.; He, X.* Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris. Journal of Pharmaceutical Analysis. 2021, 11(4), 499-504

18. Liu, J.#; He, X.#; Sun, J.; Ma, Y.* A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils. International Journal of Molecular Sciences. 2021, 22(7)

19. 李梦洁, 李占彪, 周思含, 国辉*, 何湘伟, 耿兵, 朱昌雄, 植物根际微生物调控根系构型研究, 中国农业气象. 2021, 42(11), 895-904

20. 张思琦, 王剑, 兰英, 贠娟莉, 陈建, 何湘伟*, 杜文斌*, 抗菌活性放线菌的界面微滴移液互作分离筛选方法, 微生物学通报. 2021, 48(1), 325-335

21. Cai, C.; Ma, J.; Han, C.; Jin, Y.; Zhao, G. * and He, X.*. Extraction and antioxidant activity of total triterpenoids in the mycelium of a medicinal fungus, Sanghuangporus sanghuang. Scientific Reports. 2019, 9, 7418-7427

22. Zhou，L.; Mao, S.; Huang, Q.; He, X.*and Lin, J.-M*. Inhibition of anaerobic probiotics on colorectal cancer cells using intestinal microfluidic systems, Science China. Chemistry. 2018, 61(8), 1041-1042

23. 王伟轩, 孙志朋, 霍文杰, 周琳, 王玉冰, 何湘伟*,接种高温纤维素菌剂对生活垃圾堆肥理化性质的影响, 生态科学. 2017, 36(5), 73-79

24. 王伟轩, 孙静奕, 刘伟娜, 厚凌宇, 玉王宁, 何湘伟*, 谢响明*, 微流控芯片在植物细胞研究中的应用进展, 生物技术通报. 2016, 32(6), 19-29

25. Wang, W.; Sui, Y.; Zhang, L.; Tan, W.; He, X.*, and Xiangming Xie*, Recognition of an important G-quadruplex in the HIV-1 promoter with natural small molecules, Canadian Journal of Chemistry. 2015, 94 (1), 1-6

26. He, X.; Chen, Q.; Zhang, Y.; Lin, J.-M.*, Recent advances of microchip-mass spectrometry in biological analysis. Trends in Analytical Chemistry.2014, 53, 84-97

27. He, X.; Zhang, Z.; Zhang, Q.; Yuan, G., Selective recognition of G-quadruplex in the vascular endothelial growth factor gene with small-molecule natural products by electrospray ionization (ESI) mass spectrometry and circular dichroism (CD) spectrometry. Canadian Journal of Chemistry-Revue Canadienne De Chimie. 2012, 90 (1), 55-59

28. Lin, X.; Li, H.-F.; He, X.; Hashi, Y.; Lin, J.-M.; Wang, Z., Automated online pretreatment and cleanup recycle coupled with high-performance liquid chromatography-mass spectrometry for determination of deca-bromodiphenyl ether in human serum. Journal of Separation Science. 2012, 35 (19), 2553-2558

29. Zhang, Z.; He, X.; Yuan, G., Formation and recognition of G-quadruplex relevant for pilin antigenic variation in Neisseria gonorrhoeae. Canadian Journal of Chemistry-Revue Canadienne De Chimie. 2012, 90 (1), 34-38

30. He, X.; Li, S.; Xu, M.; Lin, S.; Zhou, J.; Yuan, G., Investigation of non-covalent interaction of ANF promoter DNA and polyamides containing N-methylimidazole and N-methylpyrrole amino acids by electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry. 2011, 25 (7), 993-996.

31. Lin, S.; Li, S.; Chen, Z.; He, X.; Zhang, Y.; Xu, X.; Xu, M.; Yuan, G., Formation, recognition and bioactivities of a novel G-quadruplex in the STAT3 gene. Bioorganic & Medicinal Chemistry Letters. 2011, 21 (19), 5987-5991

32. Wu, X.; Luo, R.; He, X.; Yu, P.; Yuan, G., Letter: Electrospray ionization mass spectral characteristics and fragmentation mechanisms of triterpenoids in Fomes officinalis. European Journal of Mass Spectrometry. 2011, 17 (3), 297-304

33. Zhang, Z.; He, X.; Yuan, G., Regulation of the equilibrium between G-quadruplex and duplex DNA in promoter of human c-myc oncogene by a pyrene derivative. International Journal of Biological Macromolecules.2011, 49 (5), 1173-1176

34. He, X.; Long, H.; Yuan, G.; Xu, X.; Zhou, Y., Investigation of Interaction of Small Natural Product Molecules and Human Telomeric G-Quadruplex and Thermal Stabilities of the Complexes by Electrospray Ionization Mass Spectrometry. Acta Physico-Chimica Sinica. 2010, 26 (4), 1082-1086